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The ideal solution for removing a urine stain from a wood floor is to sand and refinish it, since it is very difficult to match up the floor finish once you have treated a urine stain in the wood. However, you can still try. To remove urine stains and odors from your wood floor, first blot the area with an absorbent cloth to remove any excess urine. The newest technology uses peroxide and detergents to penetrate the soiled area to remove the stain and deactivate the odor. Simply spray a product such as PAWS - Pet Accident Washup Solution on the soiled area, and then allow it to penetrate for approximately five minutes. Wipe clean. Another option to try is to wipe the stained area with cool water; blot dry. Next, blot the stained area with undiluted white vinegar. Wipe again with cool water and blot dry. With either of the above methods, do not saturate the wood or allow any liquid to stand on the surface for any length of time. Wipe with cool water and blot dry.
If the stain remains, you can buff with super fine 000 grade steel wool and floor wax. Rub with the grain of the wood. If the spot begins to blend with the finish, continue the wax and steel wool treatment until the appearance of the wood is restored. If the stain persists, place a paste of either pumice powder or rottenstone and vegetable oil on the spot. Using a clean, soft, absorbent cloth, gently rub the paste into the spot, following the grain of the wood. When the stain is removed, rub the surrounding area of the wood gently with the abrasive and oil paste to prevent spotting.
If the stain is still visible, the urine has probably penetrated the finish and marred the wood, in which case it is necessary to remove the finish and refinish the surface. You may be able to use super fine 000 grade steel wool and a small amount of mineral spirits to remove urine stains, but afterwards you will have to refinish the area by waxing and buffing and even then it will be difficult to match the treated area with the rest of the floor.
if your dog pees on wood floor, wipe off urine, use damp paper towels to clean floor, then use nature's miracle with a damp paper towel... then sprinkle baking soda all over the damp area where dog boobooed... let it stay there for as long as you want. It works for me all the time, absorbes odors and picks up moisture... Sweep it off, and it's like nothing happened!!
Thanks this really helped! got a great dane who went on the floor and made a huge stain. Saved me a ton of money!